Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC.
Talanta
; 85(3): 1466-71, 2011 Sep 15.
Article
in En
| MEDLINE
| ID: mdl-21807211
ABSTRACT
A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm × 50 mm+3.0 mm × 100 mm and 4.6 × 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tryptophan
/
Chromatography, High Pressure Liquid
/
Neopterin
/
Creatinine
/
Kynurenine
Limits:
Humans
Language:
En
Journal:
Talanta
Year:
2011
Type:
Article
Affiliation country:
Czech Republic