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Evaluation of homogenization techniques for the preparation of mouse tissue samples to support drug discovery.
Liang, Xiaorong; Ubhayakar, Savita; Liederer, Bianca M; Dean, Brian; Ran-Ran Qin, Ann; Shahidi-Latham, Sheerin; Deng, Yuzhong.
Affiliation
  • Liang X; Drug Metabolism & Pharmacokinetics, Genentech Inc, South San Francisco, CA 94080, USA.
Bioanalysis ; 3(17): 1923-33, 2011 Sep.
Article in En | MEDLINE | ID: mdl-21899502
BACKGROUND: In early drug-discovery research, understanding the tissue distribution of drug at the site of action can help to predict the toxicity, efficacy and exposure level of the drug. The bottleneck of tissue analysis by LC-MS/MS is the time-consuming homogenization step. RESULTS: Both mechanical and enzymatic techniques for mouse tissue homogenization were evaluated, which included bead beater, polytron and enzymatic digestion. Brain, bone marrow, kidney, spleen and liver tissues can be homogenized effectively using the bead beater alone. Lung and heart tissues were best treated with collagenase first and then homogenized by the bead beater. CONCLUSION: Homogenization conditions for seven mouse tissues have been evaluated and optimized. These findings will expedite the preparation of tissue samples for analysis.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tissue Extracts / Chromatography, Liquid / Tandem Mass Spectrometry / Drug Discovery Type of study: Evaluation_studies / Prognostic_studies Limits: Animals Language: En Journal: Bioanalysis Year: 2011 Type: Article Affiliation country: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tissue Extracts / Chromatography, Liquid / Tandem Mass Spectrometry / Drug Discovery Type of study: Evaluation_studies / Prognostic_studies Limits: Animals Language: En Journal: Bioanalysis Year: 2011 Type: Article Affiliation country: United States