[Induction of dendritic cells with multidrug resistance from K562/MDR1 cells].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 40(5): 489-94, 2011 09.
Article
in Zh
| MEDLINE
| ID: mdl-21984150
ABSTRACT
OBJECTIVE:
To induce the differentiation of K562/MDR1 cells into dendritic cells (DC) with multidrug resistance property.METHODS:
K562/MDR1 cells and K562 cells were cultured in the presence of GM-CSF and IL-4 to generate DC and matured by TNF-α. On d14 K562/MDR1-DC and K562-DC cells were harvested and the expressions of CD1a, CD83, CD80, CD86, HLA-ABC and HLA-DR were assessed by flow cytometry (FCM). The antigen presentation function of K562/MDR1-DC and K562-DC was determined by allogenic mixed lymphocyte reaction (Allo-MLR). The expression of P-glycoprotein and the intracellular accumulation of daunorubicin (DNR) were detected by FCM. The sensitivity of K562/MDR1-DC and K562-DC cell to vincristine, adriamycin was measured using MTT assay.RESULTS:
Both K562/MDR1 and K562 cells were differentiated into dendritic cells in the presence of cytokine cocktails, showing the morphologic and immunophenotypic characteristics of DC. K562/MDR1-DC more markedly enhanced proliferation of allogeneic lymphocytes in MLR than K562-DC. High level expression of P-glycoprotein and efflux of DNR were demonstrated in K562/MDR1-DC. K562/MDR1-DC showed multidrug resistance property, with higher IC(50) to VCR and ADM than that of K562-DCs.CONCLUSION:
K562/MDR1 cells can be differentiated into DC with the presence of cytokines, the induced K562/MDR1-DC cells express high level of P-glycoprotein and acquire the multidrug resistance property.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dendritic Cells
/
Cell Differentiation
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
Limits:
Humans
Language:
Zh
Journal:
Zhejiang Da Xue Xue Bao Yi Xue Ban
Journal subject:
MEDICINA
Year:
2011
Type:
Article
Affiliation country:
China