Assembly of Drosophila centromeric nucleosomes requires CID dimerization.
Mol Cell
; 45(2): 263-9, 2012 Jan 27.
Article
in En
| MEDLINE
| ID: mdl-22209075
ABSTRACT
Centromeres are essential chromosomal regions required for kinetochore assembly and chromosome segregation. The composition and organization of centromeric nucleosomes containing the essential histone H3 variant CENP-A (CID in Drosophila) is a fundamental, unresolved issue. Using immunoprecipitation of CID mononucleosomes and cysteine crosslinking, we demonstrate that centromeric nucleosomes contain CID dimers in vivo. Furthermore, CID dimerization and centromeric targeting require a residue implicated in formation of the four-helix bundle, which mediates intranucleosomal H3 dimerization and nucleosome integrity. Taken together, our findings suggest that CID nucleosomes are octameric in vivo and that CID dimerization is essential for correct centromere assembly.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Histones
/
Nucleosomes
/
Drosophila Proteins
/
DNA-Binding Proteins
/
Drosophila melanogaster
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Mol Cell
Journal subject:
BIOLOGIA MOLECULAR
Year:
2012
Type:
Article
Affiliation country:
United States