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Detection of a possible bioterrorism agent, Francisella sp., in a clinical specimen by use of next-generation direct DNA sequencing.
Kuroda, Makoto; Sekizuka, Tsuyoshi; Shinya, Fumiaki; Takeuchi, Fumihiko; Kanno, Takayuki; Sata, Tetsutaro; Asano, Shigeyuki.
Affiliation
  • Kuroda M; Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan. makokuro@nih.go.jp
J Clin Microbiol ; 50(5): 1810-2, 2012 May.
Article in En | MEDLINE | ID: mdl-22337979
ABSTRACT
Deep sequencing detected a potential bioterrorism agent, Francisella tularensis, in a human abscess sample (Iwaki-08) of unknown etiology. Identified single-nucleotide variations suggest that the Iwaki-08 case was associated with Francisella tularensis subsp. holarctica (biovar japonica) but not the highly virulent type A (Francisella tularensis subsp. tularensis).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tularemia / Abscess / High-Throughput Nucleotide Sequencing / Francisella tularensis Type of study: Diagnostic_studies Limits: Humans / Male / Middle aged Language: En Journal: J Clin Microbiol Year: 2012 Type: Article Affiliation country: Japan
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tularemia / Abscess / High-Throughput Nucleotide Sequencing / Francisella tularensis Type of study: Diagnostic_studies Limits: Humans / Male / Middle aged Language: En Journal: J Clin Microbiol Year: 2012 Type: Article Affiliation country: Japan