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Anticoagulants affect matrix metalloproteinase 9 levels in blood samples of stroke patients and healthy controls.
Rababah, Munir; Worthmann, Hans; Deb, Milani; Tryc, Anita B; Ma, Yue Tao; El Bendary, Omnia M; Hecker, Hartmut; Goldbecker, Annemarie; Heeren, Meike; Brand, Korbinian; Weissenborn, Karin; Lichtinghagen, Ralf.
Affiliation
  • Rababah M; Department of Neurology, Hannover Medical School, Carl-Neuberg-Straße 1, 30623 Hannover, Germany.
Clin Biochem ; 45(6): 483-9, 2012 Apr.
Article in En | MEDLINE | ID: mdl-22342361
OBJECTIVES: Matrix metalloproteinase-9 (MMP-9) represents a promising marker for acute stroke management. In clinical studies MMP-9 has been quantified by ELISA using differing protocols. We aimed to establish a valid protocol by evaluation of preanalytics. DESIGN AND METHODS: Blood from stroke patients (n=28) and healthy controls (n=28) was drawn into tubes containing different anticoagulants (EDTA, citrate, lithium-heparin (heparin) and heparin with proteinase inhibitors) and processed after 0, 60 and 240 min. MMP-9 plasma protein and mRNA from mononuclear leukocytes were determined. RESULTS: In regard to anticoagulants used, samples showed different MMP-9 protein baseline values and kinetics. Stable MMP-9 protein concentrations were only measured from EDTA samples. Particularly in samples with proteinase inhibitors protein and mRNA concentrations increased over time. Kinetics did not differ between patients and controls. CONCLUSIONS: Preanalytics plays a key role for determination of MMP-9. EDTA seems to be a valid anticoagulant for MMP-9 protein measurement.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Chemical Analysis / Heparin / Artifacts / Citric Acid / Stroke / Matrix Metalloproteinase 9 Type of study: Guideline / Observational_studies Limits: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Language: En Journal: Clin Biochem Year: 2012 Type: Article Affiliation country: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Chemical Analysis / Heparin / Artifacts / Citric Acid / Stroke / Matrix Metalloproteinase 9 Type of study: Guideline / Observational_studies Limits: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Language: En Journal: Clin Biochem Year: 2012 Type: Article Affiliation country: Germany