Dynex: multiplex ELISA technology.
J Lab Autom
; 17(4): 309-14, 2012 Aug.
Article
in En
| MEDLINE
| ID: mdl-22357566
ABSTRACT
Conventional enzyme-linked immunosorbent assay (ELISA) is a gold standard for screening antibodies and testing for protein or antigen presence. A significant limitation of this assay resides in the fact that only one analyte can be assessed per microplate well. Here, we describe and investigate a new technology consisting of an automated ELISA system in which up to 10 analytes can be measured within one single well, thus improving productivity, accuracy, and repeatability by reducing the amount of human labor required. Another strength of the platform is that a user can load any necessary sets/subsets of beads to perform required assays, with improved flexibility compared to manufactured-loaded arrays for multiplex analysis. We also demonstrate that this system can be used to determine the pathogenicity (i.e., presence of Shiga toxins) and serotype (i.e., Escherichia coli O157) of E. coli isolates.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Medical Laboratory Science
/
Escherichia coli O157
/
Shiga Toxins
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
J Lab Autom
Year:
2012
Type:
Article
Affiliation country:
United States