Transcriptome analysis of rosette and folding leaves in Chinese cabbage using high-throughput RNA sequencing.
Genomics
; 99(5): 299-307, 2012 May.
Article
in En
| MEDLINE
| ID: mdl-22387604
ABSTRACT
In this study, we report the first use of RNA-sequencing to gain insight into the wide range of transcriptional events that are associated with leafy head development in Chinese cabbage. We generated 53.5 million sequence reads (90 bp in length) from the rosette and heading leaves. The sequence reads were aligned to the recently sequenced Chiifu genome and were analyzed to measure the gene expression levels, to detect alternative splicing events and novel transcripts, to determine the expression of single nucleotide polymorphisms, and to refine the annotated gene structures. The analysis of the global gene expression pattern suggests two important concepts, which govern leafy head formation. Firstly, some stimuli, such as carbohydrate levels, light intensity and endogenous hormones might play a critical role in regulating the leafy head formation. Secondly, the regulation of transcription factors, protein kinases and calcium may also be involved in this developmental process.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Leaves
/
Gene Expression Profiling
/
Brassica rapa
/
High-Throughput Nucleotide Sequencing
Language:
En
Journal:
Genomics
Journal subject:
GENETICA
Year:
2012
Type:
Article
Affiliation country:
China