Noninvasive stratum corneum sampling and electron microscopical examination of skin barrier integrity: pilot study with a topical glycerin formulation for atopic dermatitis.
Skin Pharmacol Physiol
; 25(3): 155-61, 2012.
Article
in En
| MEDLINE
| ID: mdl-22399038
ABSTRACT
INTRODUCTION:
Therapy of atopic dermatitis encloses use of medicated and nonmedicated preparations. Results of clinical and biophysical procedures indirectly describe the condition of the impaired skin barrier (SB). Direct evaluation of SB integrity is only possible by electron microscopical visualization, e.g. intercellular lipid lamellae (ICLL) organization of the stratum corneum.METHOD:
SB integrity was measured by morphometric analysis of ICLL in healthy and atopic skin and after a 15-day treatment (plus 7-day follow-up) of atopic skin with a glycerin preparation.RESULTS:
Significant treatment effect was shown by the restoration of the ICLL.CONCLUSIONS:
The study reveals that morphometric analysis of ICLL organization is suitable to differentiate between healthy and diseased skin and to semiquantitatively determine the effect of a nonmedicated glycerin formulation.LIMITATION:
Small treatment cohort.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Skin Absorption
/
Microscopy, Electron, Transmission
/
Dermatitis, Atopic
/
Dermatologic Agents
/
Excipients
/
Glycerol
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Language:
En
Journal:
Skin Pharmacol Physiol
Journal subject:
DERMATOLOGIA
/
FARMACOLOGIA
/
FISIOLOGIA
Year:
2012
Type:
Article
Affiliation country:
Switzerland