Human chorionic gonadotropin interactions with immobilized anti-hCG studied by quartz-crystal microbalance with dissipation monitoring.
J Biomed Mater Res A
; 100(6): 1600-4, 2012 Jun.
Article
in En
| MEDLINE
| ID: mdl-22447558
ABSTRACT
Previous studies have found that commercial human pregnancy tests are often too insensitive to function to their advertised >99% accuracy. Improper orientation of proteins used for recognition of ligands in sensors can often prevent the binding site from being available to the ligand, thereby decreasing sensor sensitivity. We have developed a simple method for the immobilization of anti-human chorionic gonadotropin on a sensor surface that maximizes its sensitivity by ensuring ligand binding sites are exposed and densely packed. This surface also has an improved regenerative capacity over previously reported human chorionic gonadotropin sensors, retaining 99% of initial sensitivity after six regeneration cycles with 8M urea.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pregnancy Tests
/
Quartz Crystal Microbalance Techniques
/
Chorionic Gonadotropin
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
J Biomed Mater Res A
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2012
Type:
Article
Affiliation country:
United States