Using bleach-chase to measure protein half-lives in living cells. | Nat Protoc;7(4): 801-11, 2012 Mar 29. | MEDLINE

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Using bleach-chase to measure protein half-lives in living cells.

Geva-Zatorsky, Naama; Issaeva, Irina; Mayo, Avi; Cohen, Ariel; Dekel, Erez; Danon, Tamar; Cohen, Lydia; Liron, Yuvalal; Alon, Uri; Eden, Eran.

Affiliation

Geva-Zatorsky N; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

Nat Protoc ; 7(4): 801-11, 2012 Mar 29.

Article in En | MEDLINE | ID: mdl-22461069

Subject(s)

Microscopy, Fluorescence/methods; Proteins/chemistry; Proteolysis; Bacterial Proteins/analysis; Bleaching Agents/pharmacology; Genes, Reporter; Half-Life; Humans; Luminescent Proteins/analysis; Time-Lapse Imaging

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteins / Proteolysis / Microscopy, Fluorescence Limits: Humans Language: En Journal: Nat Protoc Year: 2012 Type: Article Affiliation country: Israel

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteins / Proteolysis / Microscopy, Fluorescence Limits: Humans Language: En Journal: Nat Protoc Year: 2012 Type: Article Affiliation country: Israel