Model-guided ligation strategy for optimal assembly of DNA libraries.

Ng, Daphne T W; Sarkar, Casim A

Ng, Daphne T W; Sarkar, Casim A.

Affiliation

Ng DT; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.

Protein Eng Des Sel ; 25(10): 669-78, 2012 Oct.

Article in En | MEDLINE | ID: mdl-22543356

Subject(s)

DNA/genetics; DNA/metabolism; Gene Library; Bacteriophage T4/enzymology; Base Sequence; Cloning, Molecular; DNA Ligases/metabolism; Deoxyribonucleases, Type II Site-Specific/metabolism; Directed Molecular Evolution; Genetic Vectors/genetics; Genetic Vectors/metabolism; Models, Biological; Models, Chemical; Thermodynamics

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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: DNA / Gene Library Type of study: Prognostic_studies Language: En Journal: Protein Eng Des Sel Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2012 Type: Article Affiliation country: United States

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