Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons.
Cell
; 149(7): 1635-46, 2012 Jun 22.
Article
in En
| MEDLINE
| ID: mdl-22608085
ABSTRACT
Methylation of the N(6) position of adenosine (m(6)A) is a posttranscriptional modification of RNA with poorly understood prevalence and physiological relevance. The recent discovery that FTO, an obesity risk gene, encodes an m(6)A demethylase implicates m(6)A as an important regulator of physiological processes. Here, we present a method for transcriptome-wide m(6)A localization, which combines m(6)A-specific methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-Seq). We use this method to identify mRNAs of 7,676 mammalian genes that contain m(6)A, indicating that m(6)A is a common base modification of mRNA. The m(6)A modification exhibits tissue-specific regulation and is markedly increased throughout brain development. We find that m(6)A sites are enriched near stop codons and in 3' UTRs, and we uncover an association between m(6)A residues and microRNA-binding sites within 3' UTRs. These findings provide a resource for identifying transcripts that are substrates for adenosine methylation and reveal insights into the epigenetic regulation of the mammalian transcriptome.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA Processing, Post-Transcriptional
/
Codon, Terminator
/
3' Untranslated Regions
/
Transcriptome
Type of study:
Prognostic_studies
/
Risk_factors_studies
Language:
En
Journal:
Cell
Year:
2012
Type:
Article
Affiliation country:
United States