HLA-DRß1*04 typing by simple in-house PCR-SSP technique for rheumatoid arthritis patients.
Rheumatol Int
; 33(4): 867-70, 2013 Apr.
Article
in En
| MEDLINE
| ID: mdl-22623274
ABSTRACT
A strong association between rheumatoid arthritis (RA) and human leukocyte antigen (HLA) has been observed in many different populations and that accounts for approximately one-third of the genetic component of RA susceptibility. The greatest effect comes from DRß1 gene where the strongest association has been found with DRß1*04 (DR4) allele. As serology has some disadvantages over polymerase chain reaction (PCR)-based techniques and commercially available PCR-based kits are expensive, this study was aimed to standardize simple in-house PCR-SSP technique. Accuracy of this test was further checked with standard PCR-SSOP (RLS) results. The frequency HLA-DRß1*04 was significantly increased among RA patients when compared with normal controls. In this study, a very simple, convenient and more cost-effective in-house PCR-SSP technique was standardized for HLA-DRß1*04 typing that is helpful to RA diagnosis in developing countries like India, which can be used as a good screening test.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arthritis, Rheumatoid
/
Histocompatibility Testing
/
HLA-DRB1 Chains
Type of study:
Diagnostic_studies
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Country/Region as subject:
Asia
Language:
En
Journal:
Rheumatol Int
Year:
2013
Type:
Article
Affiliation country:
India