High-content live-cell imaging assay used to establish mechanism of trastuzumab emtansine (T-DM1)--mediated inhibition of platelet production.

Thon, Jonathan N; Devine, Matthew T; Jurak Begonja, Antonija; Tibbitts, Jay; Italiano, Joseph E

Thon, Jonathan N; Devine, Matthew T; Jurak Begonja, Antonija; Tibbitts, Jay; Italiano, Joseph E.

Affiliation

Thon JN; Hematology Division, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.

Blood ; 120(10): 1975-84, 2012 Sep 06.

Article in En | MEDLINE | ID: mdl-22665936

Subject(s)

Antibodies, Monoclonal, Humanized/pharmacology; Blood Platelets/drug effects; Cell Differentiation/drug effects; Maytansine/analogs & derivatives; Megakaryocytes/drug effects; Thrombocytopenia/chemically induced; Ado-Trastuzumab Emtansine; Animals; Biological Assay; Blood Platelets/physiology; Blood Platelets/ultrastructure; Cell Differentiation/physiology; Fetus; Flow Cytometry; Humans; Liver/cytology; Liver/drug effects; Liver/physiology; Maytansine/pharmacology; Megakaryocytes/physiology; Megakaryocytes/ultrastructure; Mice; Microscopy, Fluorescence; Microtubules/drug effects; Microtubules/ultrastructure; Molecular Imaging; Platelet Count; Primary Cell Culture; Thrombocytopenia/prevention & control; Thrombopoiesis/drug effects; Thrombopoiesis/physiology; Trastuzumab; Tubulin/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Thrombocytopenia / Blood Platelets / Megakaryocytes / Cell Differentiation / Antibodies, Monoclonal, Humanized / Maytansine Type of study: Qualitative_research Limits: Animals / Humans Language: En Journal: Blood Year: 2012 Type: Article Affiliation country: United States

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