The pan-genotype specificity of the hepatitis C virus anti-core monoclonal antibody C7-50 is contingent on the quasispecies profile of a population.
Arch Virol
; 157(11): 2235-9, 2012 Nov.
Article
in En
| MEDLINE
| ID: mdl-22828781
ABSTRACT
The inter/intra-genotype quasispecies makeup of hepatitis C virus (HCV) has retarded the development of antibodies capable of pan-genotype reactivity. Mutations, even in conserved domains, are tolerated to a degree. In this report, we characterise the pan-genotype specificity of the commercially available monoclonal anti-HCV core antibody C7-50. We demonstrate the antibody's ability to detect HCV core protein following infection of Huh7 cells with serum-derived HCV of genotypes 2-5 and that a single-site polymorphism in a genotype 3a core amino acid sequence is sufficient to disrupt antibody recognition of the epitope. This same polymorphism is a feature of genotype 3 viruses.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Core Proteins
/
Hepacivirus
/
Hepatitis C Antibodies
/
Polymorphism, Single Nucleotide
/
Antibodies, Monoclonal
Limits:
Humans
Language:
En
Journal:
Arch Virol
Year:
2012
Type:
Article
Affiliation country:
Ireland