Assay development for identifying inhibitors of the mycobacterial FadD32 activity.

Galandrin, Ségolène; Guillet, Valérie; Rane, Rajendra S; Léger, Mathieu; N, Radha; Eynard, Nathalie; Das, Kaveri; Balganesh, Tanjore S; Mourey, Lionel; Daffé, Mamadou; Marrakchi, Hedia

Galandrin, Ségolène; Guillet, Valérie; Rane, Rajendra S; Léger, Mathieu; N, Radha; Eynard, Nathalie; Das, Kaveri; Balganesh, Tanjore S; Mourey, Lionel; Daffé, Mamadou; Marrakchi, Hedia.

Affiliation

Galandrin S; Institut de Pharmacologie et de Biologie Structurale (IPBS), Centre National de la Recherche Scientifique (CNRS), Département "Mécanismes Moléculaires des Infections Mycobactériennes," Toulouse, France.

J Biomol Screen ; 18(5): 576-87, 2013 Jun.

Article in En | MEDLINE | ID: mdl-23364516

Subject(s)

Antitubercular Agents/isolation & purification; High-Throughput Screening Assays/methods; Ligases/antagonists & inhibitors; Mycobacterium tuberculosis/drug effects; Antitubercular Agents/pharmacology; Chromatography, Thin Layer/methods; Drug Discovery/methods; Ligases/genetics; Ligases/metabolism; Models, Biological; Mycobacterium smegmatis/enzymology; Mycobacterium smegmatis/genetics; Mycobacterium smegmatis/metabolism; Mycobacterium tuberculosis/enzymology; Mycobacterium tuberculosis/genetics; Mycolic Acids/metabolism; Protein Binding; Recombinant Proteins/antagonists & inhibitors; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Substrate Specificity; Validation Studies as Topic

Key words

FAAL; assay adaptation; fatty acyl-AMP ligase; mycobacteria; screening of inhibitors

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: High-Throughput Screening Assays / Ligases / Mycobacterium tuberculosis / Antitubercular Agents Language: En Journal: J Biomol Screen Journal subject: BIOLOGIA MOLECULAR Year: 2013 Type: Article Affiliation country: France

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