Multiplex agarose gel electrophoresis system for variable number of tandem repeats genotyping: analysis example using Mycobacterium tuberculosis.
Electrophoresis
; 34(8): 1171-4, 2013 Apr.
Article
in En
| MEDLINE
| ID: mdl-23401033
ABSTRACT
As one genotyping method for Mycobacterium tuberculosis, variable number of tandem repeats (VNTR) is a promising tool to trace the undefined transmission of tuberculosis, but it often requires large equipment such as a genetic analyzer for DNA fragment analysis or CE system to conduct systematic analyses. For convenient genotyping at low cost in laboratories, we designed a multiplex PCR system that is applicable to agarose gel electrophoresis using fluorescent PCR primers. For tuberculosis genotyping by VNTR, the copy quantities of minisatellite DNA must be determined in more than 12 loci. The system can halve laborious electrophoresis processes by presenting an image of two VNTR amplicons on a single lane. No expensive equipment is necessary for this method. Therefore, it is useful even in developing countries.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Minisatellite Repeats
/
Electrophoresis, Agar Gel
/
Genotyping Techniques
/
Multiplex Polymerase Chain Reaction
/
Mycobacterium tuberculosis
Limits:
Humans
Language:
En
Journal:
Electrophoresis
Year:
2013
Type:
Article
Affiliation country:
Japan