Bis-(3'-5')-cyclic dimeric GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa.
Antimicrob Agents Chemother
; 57(5): 2066-75, 2013 May.
Article
in En
| MEDLINE
| ID: mdl-23403434
ABSTRACT
Bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) is an intracellular second messenger that controls the lifestyles of many bacteria. A high intracellular level of c-di-GMP induces a biofilm lifestyle, whereas a low intracellular level of c-di-GMP stimulates dispersal of biofilms and promotes a planktonic lifestyle. Here, we used the expression of different reporters to show that planktonic cells, biofilm cells, and cells dispersed from biofilms (DCells) had distinct intracellular c-di-GMP levels. Proteomics analysis showed that the low intracellular c-di-GMP level of DCells induced the expression of proteins required for the virulence and development of antimicrobial peptide resistance in Pseudomonas aeruginosa. In accordance with this, P. aeruginosa cells with low c-di-GMP levels were found to be more resistant to colistin than P. aeruginosa cells with high c-di-GMP levels. This finding contradicts the current dogma stating that dispersed cells are inevitably more susceptible to antibiotics than their sessile counterparts.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudomonas aeruginosa
/
Bacterial Proteins
/
Second Messenger Systems
/
Gene Expression Regulation, Bacterial
/
Colistin
/
Cyclic GMP
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Drug Resistance, Bacterial
/
Anti-Bacterial Agents
Language:
En
Journal:
Antimicrob Agents Chemother
Year:
2013
Type:
Article
Affiliation country:
Singapore