A simple and effective pressure culture system modified from a transwell cell culture system.
Biol Res
; 46(1): 47-52, 2013.
Article
in En
| MEDLINE
| ID: mdl-23760414
ABSTRACT
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin ß1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Culture Techniques
/
Integrin beta1
/
Cell Proliferation
Limits:
Animals
/
Humans
Language:
En
Journal:
Biol Res
Journal subject:
BIOLOGIA
Year:
2013
Type:
Article
Affiliation country:
China