CryoEM and molecular dynamics of the circadian KaiB-KaiC complex indicates that KaiB monomers interact with KaiC and block ATP binding clefts.

Villarreal, Seth A; Pattanayek, Rekha; Williams, Dewight R; Mori, Tetsuya; Qin, Ximing; Johnson, Carl H; Egli, Martin; Stewart, Phoebe L

Villarreal, Seth A; Pattanayek, Rekha; Williams, Dewight R; Mori, Tetsuya; Qin, Ximing; Johnson, Carl H; Egli, Martin; Stewart, Phoebe L.

Affiliation

Villarreal SA; Department of Pharmacology and Cleveland Center for Membrane and Structural Biology, Case Western Reserve University, Cleveland, OH 44106, USA.

J Mol Biol ; 425(18): 3311-24, 2013 Sep 23.

Article in En | MEDLINE | ID: mdl-23796516

Subject(s)

Adenosine Triphosphate/metabolism; Bacterial Proteins/metabolism; Circadian Rhythm Signaling Peptides and Proteins/metabolism; Synechococcus/metabolism; Bacterial Proteins/antagonists & inhibitors; Bacterial Proteins/chemistry; Binding Sites/physiology; Circadian Rhythm Signaling Peptides and Proteins/antagonists & inhibitors; Circadian Rhythm Signaling Peptides and Proteins/chemistry; Cryoelectron Microscopy; Crystallography, X-Ray; Models, Molecular; Molecular Dynamics Simulation; Multiprotein Complexes/chemistry; Phosphorylation; Protein Binding; Protein Multimerization/physiology; Protein Structure, Quaternary; Synechococcus/genetics

Key words

EM; FSC; Fourier shell correlation; GST; MDFF; PDB; PTO; Protein Data Bank; SAXS; Synechococcus elongatus; circadian oscillator; cryo-electron microscopy; cryoEM; electron microscopy; glutathione S-transferase; molecular dynamics flexible fitting; posttranslational oscillator; proteinprotein interface; small-angle X-ray scattering

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Adenosine Triphosphate / Synechococcus / Circadian Rhythm Signaling Peptides and Proteins Type of study: Prognostic_studies Language: En Journal: J Mol Biol Year: 2013 Type: Article Affiliation country: United States

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