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Epigenetic and transcriptional signatures of stable versus plastic differentiation of proinflammatory γδ T cell subsets.
Schmolka, Nina; Serre, Karine; Grosso, Ana R; Rei, Margarida; Pennington, Daniel J; Gomes, Anita Q; Silva-Santos, Bruno.
Affiliation
  • Schmolka N; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
  • Serre K; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
  • Grosso AR; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
  • Rei M; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
  • Pennington DJ; Blizard Institute, Barts and The London School of Medicine, Queen Mary University of London, UK.
  • Gomes AQ; Blizard Institute, Barts and The London School of Medicine, Queen Mary University of London, UK.
  • Silva-Santos B; Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
Nat Immunol ; 14(10): 1093-1100, 2013 Oct.
Article in En | MEDLINE | ID: mdl-23995235
Two distinct subsets of γδ T cells that produce interleukin 17 (IL-17) (CD27(-) γδ T cells) or interferon-γ (IFN-γ) (CD27(+) γδ T cells) develop in the mouse thymus, but the molecular determinants of their functional potential in the periphery remain unknown. Here we conducted a genome-wide characterization of the methylation patterns of histone H3, along with analysis of mRNA encoding transcription factors, to identify the regulatory networks of peripheral IFN-γ-producing or IL-17-producing γδ T cell subsets in vivo. We found that CD27(+) γδ T cells were committed to the expression of Ifng but not Il17, whereas CD27(-) γδ T cells displayed permissive chromatin configurations at loci encoding both cytokines and their regulatory transcription factors and differentiated into cells that produced both IL-17 and IFN-γ in a tumor microenvironment.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Differentiation / T-Lymphocyte Subsets / Receptors, Antigen, T-Cell, gamma-delta / Gene Expression Profiling / Epigenesis, Genetic / Transcriptome Type of study: Prognostic_studies Limits: Animals Language: En Journal: Nat Immunol Journal subject: ALERGIA E IMUNOLOGIA Year: 2013 Type: Article Affiliation country: Portugal

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Differentiation / T-Lymphocyte Subsets / Receptors, Antigen, T-Cell, gamma-delta / Gene Expression Profiling / Epigenesis, Genetic / Transcriptome Type of study: Prognostic_studies Limits: Animals Language: En Journal: Nat Immunol Journal subject: ALERGIA E IMUNOLOGIA Year: 2013 Type: Article Affiliation country: Portugal