Sortase-tag expressed protein ligation: combining protein purification and site-specific bioconjugation into a single step.

Warden-Rothman, Robert; Caturegli, Ilaria; Popik, Vladimir; Tsourkas, Andrew

Warden-Rothman, Robert; Caturegli, Ilaria; Popik, Vladimir; Tsourkas, Andrew.

Affiliation

Warden-Rothman R; Department of Bioengineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.

Anal Chem ; 85(22): 11090-7, 2013 Nov 19.

Article in En | MEDLINE | ID: mdl-24111659

Subject(s)

Aminoacyltransferases/chemistry; Azides/chemistry; Bacterial Proteins/chemistry; Cysteine Endopeptidases/chemistry; Green Fluorescent Proteins/metabolism; Magnetic Resonance Imaging/methods; Nanoparticles/chemistry; Receptor, ErbB-2/analysis; Animals; Blotting, Western; Click Chemistry; Fluorescence; Mice; Peptide Fragments/metabolism; Receptor, ErbB-2/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Azides / Bacterial Proteins / Cysteine Endopeptidases / Magnetic Resonance Imaging / Receptor, ErbB-2 / Aminoacyltransferases / Green Fluorescent Proteins / Nanoparticles Limits: Animals Language: En Journal: Anal Chem Year: 2013 Type: Article Affiliation country: United States

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