Labeling of the pathogenic bacterium Staphylococcus aureus with gold or ferric oxide-core nanoparticles highlights new capabilities for investigation of host-pathogen interactions.

Depke, Maren; Surmann, Kristin; Hildebrandt, Petra; Jehmlich, Nico; Michalik, Stephan; Stanca, Sarmiza E; Fritzsche, Wolfgang; Völker, Uwe; Schmidt, Frank

Depke, Maren; Surmann, Kristin; Hildebrandt, Petra; Jehmlich, Nico; Michalik, Stephan; Stanca, Sarmiza E; Fritzsche, Wolfgang; Völker, Uwe; Schmidt, Frank.

Affiliation

Depke M; ZIK-FunGene Junior Research Group "Applied Proteomics," Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.

Cytometry A ; 85(2): 140-50, 2014 Feb.

Article in En | MEDLINE | ID: mdl-24347542

Subject(s)

Ferric Compounds/chemistry; Gold/chemistry; Host-Pathogen Interactions; Metal Nanoparticles/chemistry; Staining and Labeling/methods; Staphylococcus aureus/growth & development; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Line; Epithelial Cells/cytology; Epithelial Cells/microbiology; Flow Cytometry; Fluorescent Dyes/chemistry; Gene Expression; Humans; Magnets; Microscopy, Fluorescence; Polyvinyl Alcohol/chemistry; Proteome/genetics; Proteome/metabolism; Staphylococcus aureus/ultrastructure

Key words

SILAC; Staphylococcus aureus; bacterial labeling; cell culture infection model; fluorescence microscopy; fluorescence-activated cell sorting; host-pathogen interaction; magnetic separation; mass spectrometry; nanoparticle; proteomics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staining and Labeling / Staphylococcus aureus / Ferric Compounds / Metal Nanoparticles / Host-Pathogen Interactions / Gold Limits: Humans Language: En Journal: Cytometry A Year: 2014 Type: Article Affiliation country: Germany

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