Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.

Varga, E; Nemes, C; Davis, R P; Ujhelly, O; Klincumhom, N; Polgar, Z; Muenthaisong, S; Pirity, M K; Dinnyes, A

Varga, E; Nemes, C; Davis, R P; Ujhelly, O; Klincumhom, N; Polgar, Z; Muenthaisong, S; Pirity, M K; Dinnyes, A.

Affiliation

Varga E; Molecular Animal Biotechnology Laboratory, Szent István University, 2100 Gödöllo, Hungary; BioTalentum Ltd., Aulich Lajos Street 26, 2100 Gödöllo, Hungary.
Nemes C; BioTalentum Ltd., Aulich Lajos Street 26, 2100 Gödöllo, Hungary.
Davis RP; Department of Anatomy & Embryology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
Ujhelly O; BioTalentum Ltd., Aulich Lajos Street 26, 2100 Gödöllo, Hungary.
Klincumhom N; BioTalentum Ltd., Aulich Lajos Street 26, 2100 Gödöllo, Hungary; Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 10700 Bangkok, Thailand.
Polgar Z; BioTalentum Ltd., Aulich Lajos Street 26, 2100 Gödöllo, Hungary.
Muenthaisong S; BioTalentum Ltd., Aulich Lajos Street 26, 2100 Gödöllo, Hungary; Department of Farm Animal Health and Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands.
Pirity MK; BioTalentum Ltd., Aulich Lajos Street 26, 2100 Gödöllo, Hungary.
Dinnyes A; Molecular Animal Biotechnology Laboratory, Szent István University, 2100 Gödöllo, Hungary; BioTalentum Ltd., Aulich Lajos Street 26, 2100 Gödöllo, Hungary; Department of Farm Animal Health and Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Net

Exp Cell Res ; 322(2): 335-44, 2014 Apr 01.

Article in En | MEDLINE | ID: mdl-24560743

Subject(s)

Cell Differentiation; Cellular Reprogramming; Genetic Vectors; Induced Pluripotent Stem Cells/cytology; Induced Pluripotent Stem Cells/metabolism; Lentivirus/genetics; Alkaline Phosphatase/genetics; Alkaline Phosphatase/metabolism; Animals; Blotting, Southern; Blotting, Western; Cell Proliferation; Cells, Cultured; Electroporation; Embryo, Mammalian/cytology; Embryo, Mammalian/metabolism; Embryonic Stem Cells/cytology; Embryonic Stem Cells/metabolism; Fibroblasts/cytology; Fibroblasts/metabolism; Flow Cytometry; HEK293 Cells; Heart/embryology; Heart/physiology; Humans; Immunoenzyme Techniques; Integrases/metabolism; Mice; Mice, Inbred C57BL; Neurons/cytology; Neurons/metabolism; RNA, Messenger/genetics; Real-Time Polymerase Chain Reaction; Recombination, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Transduction, Genetic; Transgenes

Key words

Differentiation; Germ-line; Mouse; Pluripotent; Reprogramming; Stem cell

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Differentiation / Lentivirus / Cellular Reprogramming / Induced Pluripotent Stem Cells / Genetic Vectors Language: En Journal: Exp Cell Res Year: 2014 Type: Article Affiliation country: Hungary

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