Alteration of amino-terminal codons of human granulocyte-colony-stimulating factor increases expression levels and allows efficient processing by methionine aminopeptidase in Escherichia coli.

Devlin, P E; Drummond, R J; Toy, P; Mark, D F; Watt, K W; Devlin, J J

Devlin, P E; Drummond, R J; Toy, P; Mark, D F; Watt, K W; Devlin, J J.

Affiliation

Devlin PE; Department of Molecular Biology, Cetus Corporation, Emeryville, CA 94608.

Gene ; 65(1): 13-22, 1988 May 15.

Article in En | MEDLINE | ID: mdl-2456256

Subject(s)

Aminopeptidases/metabolism; Colony-Stimulating Factors/genetics; Granulocytes/metabolism; Protein Processing, Post-Translational; Base Sequence; Codon; Colony-Stimulating Factors/biosynthesis; Colony-Stimulating Factors/isolation & purification; DNA/genetics; Escherichia coli/genetics; Genetic Vectors; Granulocyte Colony-Stimulating Factor; Humans; Methionyl Aminopeptidases; Molecular Sequence Data; RNA, Messenger/genetics; Recombinant Proteins/biosynthesis; Recombinant Proteins/isolation & purification; Transcription, Genetic

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Collection: 01-internacional Database: MEDLINE Main subject: Protein Processing, Post-Translational / Colony-Stimulating Factors / Granulocytes / Aminopeptidases Type of study: Prognostic_studies Limits: Humans Language: En Journal: Gene Year: 1988 Type: Article

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