Antigenic sites in carcinoembryonic antigen.
Cancer Res
; 49(17): 4852-8, 1989 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-2474375
ABSTRACT
The epitope reactivities of 52 well-characterized monoclonal antibodies (Mabs) against carcinoembryonic antigen from 11 different research groups were studied using competitive solid-phase immunoassays. About 60% of all possible combinations of Mabs as inhibitors and as the primary binding antibody were investigated. The inhibition data were analyzed by a specially developed computer program "EPITOPES" which measures concordance and discordance in inhibition patterns between Mabs. The analysis showed that 43 of the 52 Mabs (83%) could be classified into one of five essentially noninteracting epitope groups (GOLD 1-5) containing between four and 15 Mabs each. The epitopes recognized by the Mabs belonging to groups 1 to 5 were peptide in nature. With one or two possible exceptions non-classifiable Mabs were either directed against carbohydrate epitopes (4 Mabs) or were inactive in the tests used. Within epitope groups GOLD 1, 4, and 5 two partially overlapping subgroups were distinguished. Mabs with a high degree of carcinoembryonic antigen specificity generally belonged to epitope groups GOLD 1 and 3.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carcinoembryonic Antigen
/
Epitopes
Limits:
Humans
Language:
En
Journal:
Cancer Res
Year:
1989
Type:
Article