Human vascular tissue models formed from human induced pluripotent stem cell derived endothelial cells.
Stem Cell Rev Rep
; 11(3): 511-25, 2015 Jun.
Article
in En
| MEDLINE
| ID: mdl-25190668
ABSTRACT
Here we describe a strategy to model blood vessel development using a well-defined induced pluripotent stem cell-derived endothelial cell type (iPSC-EC) cultured within engineered platforms that mimic the 3D microenvironment. The iPSC-ECs used here were first characterized by expression of endothelial markers and functional properties that included VEGF responsiveness, TNF-α-induced upregulation of cell adhesion molecules (MCAM/CD146; ICAM1/CD54), thrombin-dependent barrier function, shear stress-induced alignment, and 2D and 3D capillary-like network formation in Matrigel. The iPSC-ECs also formed 3D vascular networks in a variety of engineering contexts, yielded perfusable, interconnected lumen when co-cultured with primary human fibroblasts, and aligned with flow in microfluidics devices. iPSC-EC function during tubule network formation, barrier formation, and sprouting was consistent with that of primary ECs, and the results suggest a VEGF-independent mechanism for sprouting, which is relevant to therapeutic anti-angiogenesis strategies. Our combined results demonstrate the feasibility of using a well-defined, stable source of iPSC-ECs to model blood vessel formation within a variety of contexts using standard in vitro formats.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Vessels
/
Cell Differentiation
/
Neovascularization, Physiologic
/
Induced Pluripotent Stem Cells
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Stem Cell Rev Rep
Year:
2015
Type:
Article
Affiliation country:
United States