The Pael-R gene does not mediate the changes in rotenone-induced Parkinson's disease model cells.
Neural Regen Res
; 9(4): 402-6, 2014 Feb 15.
Article
in En
| MEDLINE
| ID: mdl-25206827
ABSTRACT
In this study, we established cell models for Parkinson's disease using rotenone. An RNA interference vector targeting Parkin-associated endothelin receptor-like receptor (Pael-R) was transfected into the model cells. The results of reverse-transcription polymerase chain reaction and western blot analysis showed that Pael-R expression was decreased after RNA interference compared with the control group (no treatment) and the model group (rotenone treatment), while the rate of apoptosis and survival of dopaminergic cells did not differ significantly between groups, as detected by flow cytometry and an MTT assay. These experimental findings indicate that the Pael-R gene has no role in the changes in rotenone-induced Parkinson's disease model cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Prognostic_studies
Language:
En
Journal:
Neural Regen Res
Year:
2014
Type:
Article
Affiliation country:
China