Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits.

Boes, Alexander; Spiegel, Holger; Edgue, Gueven; Kapelski, Stephanie; Scheuermayer, Matthias; Fendel, Rolf; Remarque, Edmond; Altmann, Friedrich; Maresch, Daniel; Reimann, Andreas; Pradel, Gabriele; Schillberg, Stefan; Fischer, Rainer

Boes, Alexander; Spiegel, Holger; Edgue, Gueven; Kapelski, Stephanie; Scheuermayer, Matthias; Fendel, Rolf; Remarque, Edmond; Altmann, Friedrich; Maresch, Daniel; Reimann, Andreas; Pradel, Gabriele; Schillberg, Stefan; Fischer, Rainer.

Affiliation

Boes A; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany.

Plant Biotechnol J ; 13(2): 222-34, 2015 Feb.

Article in En | MEDLINE | ID: mdl-25236489

Subject(s)

Antigens, Protozoan/metabolism; Malaria Vaccines/immunology; Membrane Proteins/metabolism; Nicotiana/genetics; Plasmodium falciparum/growth & development; Protozoan Proteins/metabolism; Animals; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Glycosylation; Immune Sera; Immunization; Immunoglobulin G/metabolism; Merozoites/metabolism; Models, Molecular; Parasites/metabolism; Pichia; Plants, Genetically Modified; Polysaccharides/metabolism; Rabbits; Surface Plasmon Resonance

Key words

Malaria; Plasmodium falciparum; glycosylation; plants; transient expression; vaccine

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasmodium falciparum / Nicotiana / Protozoan Proteins / Malaria Vaccines / Membrane Proteins / Antigens, Protozoan Limits: Animals Language: En Journal: Plant Biotechnol J Journal subject: BIOTECNOLOGIA / BOTANICA Year: 2015 Type: Article Affiliation country: Germany

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