Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil.
Arch Virol
; 160(1): 21-7, 2015 Jan.
Article
in En
| MEDLINE
| ID: mdl-25252815
ABSTRACT
We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Culex
/
Flavivirus
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Arch Virol
Year:
2015
Type:
Article