Comparison of multilocus sequence typing, RAPD, and MALDI-TOF mass spectrometry for typing of ß-lactam-resistant Klebsiella pneumoniae strains.
Diagn Microbiol Infect Dis
; 80(4): 267-71, 2014 Dec.
Article
in En
| MEDLINE
| ID: mdl-25266674
ABSTRACT
Extended spectrum of ß-lactam (ESBL) resistance of Klebsiella pneumoniae has become an increasing problem in hospital infections. Typing of isolates is important to establish the intrahospital surveillance of resistant clones. In this study, the discriminatory potential of randomly amplified polymorphic DNA and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analyses were compared with multilocus sequence typing (MLST) by using 17 ß-lactam-resistant K. pneumoniae isolates of different genotypes. MLST alleles were distributed in 8 sequence types (STs). Among ESBL strains of the same ST, the presence of different ß-lactamase genes was common. RAPD band patterns also revealed 8 types that corresponded to MLST-defined genotypes in 15 out of 17 cases. MALDI-TOF analysis could differentiate 5 clusters of strains. The results of this work show that RAPD may be usable as a rapid screening method for the intrahospital surveillance of K. pneumoniae, allowing a discrimination of clonally related strains. MALDI-TOF-based typing was not strongly corresponding to genotyping and warrants further investigation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Random Amplified Polymorphic DNA Technique
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Multilocus Sequence Typing
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Klebsiella pneumoniae
Limits:
Humans
Language:
En
Journal:
Diagn Microbiol Infect Dis
Year:
2014
Type:
Article
Affiliation country:
Germany