D2-like receptor activation does not initiate a brain docosahexaenoic acid signal in unanesthetized rats.
BMC Neurosci
; 15: 113, 2014 Oct 30.
Article
in En
| MEDLINE
| ID: mdl-25359512
ABSTRACT
BACKGROUND:
The polyunsaturated fatty acid, docosahexaenoic acid (DHA), participates in neurotransmission involving activation of calcium-independent phospholipase A2 (iPLA2), which is coupled to muscarinic, cholinergic and serotonergic neuroreceptors. Drug induced activation of iPLA2 can be measured in vivo with quantitative autoradiography using 14C-DHA as a probe. The present study used this approach to address whether a DHA signal is produced following dompaminergic (D)2-like receptor activation with quinpirole in rat brain. Unanesthetized rats were infused intravenously with 14C-DHA one minute after saline or quinpirole infusion, and serial blood samples were collected over a 20-minute period to obtain plasma. The animals were euthanized with sodium pentobarbital and their brains excised, coronally dissected and subjected to quantitative autoradiography to derive the regional incorporation coefficient, k*, a marker of DHA signaling. Plasma labeled and unlabeled unesterified DHA concentrations were measured.RESULTS:
The incorporation coefficient (k*) for DHA did not differ significantly between quinpirole-treated and control rats in any of 81 identified brain regions. Plasma labeled DHA concentration over the 20-minute collection period (input function) and unlabeled unesterified DHA concentration did not differ significantly between the two groups.CONCLUSION:
These findings demonstrate that D2-like receptor initiated signaling does not involve DHA as a second messenger, and likely does not involve iPLA2 activation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain
/
Docosahexaenoic Acids
/
Dopamine Agonists
/
Quinpirole
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
BMC Neurosci
Journal subject:
NEUROLOGIA
Year:
2014
Type:
Article
Affiliation country:
United States