Human interferon regulatory factor 5 homologous epitopes of Epstein-Barr virus and Mycobacterium avium subsp. paratuberculosis induce a specific humoral and cellular immune response in multiple sclerosis patients.
Mult Scler
; 21(8): 984-95, 2015 Jul.
Article
in En
| MEDLINE
| ID: mdl-25392335
ABSTRACT
BACKGROUND:
A large number of reports indicate the association of Epstein-Barr virus (EBV), and Mycobacterium avium subsp. paratuberculosis (MAP) with multiple sclerosis (MS).OBJECTIVE:
To gain a better understanding of the role of these two pathogens, we investigated the host response induced by selected antigenic peptides.METHODS:
We examined both humoral and cell-mediated responses against peptides deriving from EBV tegument protein BOLF1, the MAP_4027 and the human interferon regulatory factor 5 (IRF5424-434) homolog in several MS patients and healthy controls (HCs).RESULTS:
Antibodies against these peptides were highly prevalent in MS patients compared to HCs. Concerning MS patients, BOLF1305-320, MAP_402718-32 and IRF5424-434 peptides were able to induce mainly Th1-related cytokines secretion, whereas Th2-related cytokines were down-regulated. Flow cytometry analyses performed on a subset of MS patients highlighted that these peptides were capable of inducing the release of pro-inflammatory cytokines IFN-γ and TNF-α by CD4(+) and CD8(+) T lymphocytes, and IL-6 and TNF-α by CD14(+) monocyte cells.CONCLUSION:
Our data demonstrated that both EBV and MAP epitopes elicit a consistent humoral response in MS patients compared to HCs, and that the aforementioned peptides are able to induce a T-cell-mediated response that is MS correlated.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mycobacterium avium subsp. paratuberculosis
/
Herpesvirus 4, Human
/
Interferon Regulatory Factors
/
Immunity, Humoral
/
Immunity, Cellular
/
Multiple Sclerosis
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Mult Scler
Journal subject:
NEUROLOGIA
Year:
2015
Type:
Article
Affiliation country:
Italy