High-throughput chemical modification of oligonucleotides for systematic structure-activity relationship evaluation.
Bioconjug Chem
; 25(12): 2222-32, 2014 Dec 17.
Article
in En
| MEDLINE
| ID: mdl-25398098
ABSTRACT
Chemical modification of siRNA is achieved in a high-throughput manner (96-well plate format) by copper catalyzed azide-alkyne cycloadditions. This transformation can be performed in one synthetic operation at up to four positions with complete specificity, good yield, and acceptable purity. As demonstrated here, this approach extends the current synthetic options for oligonucleotide modifications and simultaneously facilitates the systematic, rapid biological evaluation of modified siRNA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotides
/
Structure-Activity Relationship
/
High-Throughput Screening Assays
Limits:
Humans
Language:
En
Journal:
Bioconjug Chem
Journal subject:
BIOQUIMICA
Year:
2014
Type:
Article
Affiliation country:
United States