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Impact of natural IgM concentration on gene therapy with adenovirus type 5 vectors.
Qiu, Qi; Xu, Zhili; Tian, Jie; Moitra, Rituparna; Gunti, Sreenivasulu; Notkins, Abner L; Byrnes, Andrew P.
Affiliation
  • Qiu Q; Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
  • Xu Z; Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
  • Tian J; Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
  • Moitra R; Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
  • Gunti S; Experimental Medicine Section, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland, USA.
  • Notkins AL; Experimental Medicine Section, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland, USA.
  • Byrnes AP; Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA Andrew.Byrnes@FDA.HHS.gov.
J Virol ; 89(6): 3412-6, 2015 Mar.
Article in En | MEDLINE | ID: mdl-25552715
ABSTRACT
Natural IgM inhibits gene transfer by adenovirus type 5 (Ad5) vectors. We show that polyreactive natural IgM antibodies bind to Ad5 and that inhibition of liver transduction by IgM depends on Kupffer cells. By manipulating IgM concentration in vivo, we demonstrate that IgM inhibits liver transduction in a concentration-dependent manner. We further show that differences in natural IgM between BALB/c and C57BL/6 mice contribute to lower efficiency of Ad5 gene transfer in BALB/c mice.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Immunoglobulin M / Genetic Therapy / Adenoviridae / Genetic Vectors Limits: Animals Language: En Journal: J Virol Year: 2015 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Immunoglobulin M / Genetic Therapy / Adenoviridae / Genetic Vectors Limits: Animals Language: En Journal: J Virol Year: 2015 Type: Article Affiliation country: United States