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Chondrocyte clusters adjacent to sites of cartilage degeneration have characteristics of progenitor cells.
Hoshiyama, Yoshiaki; Otsuki, Shuhei; Oda, Shuhei; Kurokawa, Yoshitaka; Nakajima, Mikio; Jotoku, Tsuyoshi; Tamura, Ryuichi; Okamoto, Yoshinori; Lotz, Martin K; Neo, Masashi.
Affiliation
  • Hoshiyama Y; Department of Orthopedic Surgery, Osaka Medical College, 2-7 Daigakumachi, Osaka, 569-8686, Japan.
J Orthop Res ; 33(4): 548-55, 2015 Apr.
Article in En | MEDLINE | ID: mdl-25691232
The purpose of this study was to investigate the site-specific characteristics and roles of chondrocyte clusters in human knee osteoarthritis. Cartilage explants were obtained from 45 knees undergoing total knee replacement surgery. The explants were taken from 4 locations in the knee: the medial femoral condyle, the medial posterior femoral condyle (MPC), the lateral femoral condyle, and the lateral posterior femoral condyle (LPC). Cartilage degeneration, cell density, and cell arrangement were compared histologically. A live/dead cell viability assay and immunohistochemical analyses using antibodies against STRO-1, FGF2, and Ki-67 were performed. Cell proliferation and cartilaginous nodule production in MPC and LPC explants in monolayer culture were compared. Finally, MPC cartilage explants were cultured to observe histological changes. The cell density of the MPC explants was higher than that of the LPC because of clustering. MPC explants contained more live cells than the LPC did, and the expression of IHC markers in MPC explants was higher than that in LPC. Chondrocytes from MPC proliferated faster and produced more nodules in monolayer culture than those from the LPC and MPC explants were repaired during organ culture. In conclusion, chondrocyte clusters adjacent to severe cartilage degeneration have specific characteristics, with progenitor and proliferative potential.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stem Cells / Cartilage, Articular / Bone Malalignment / Chondrocytes / Osteoarthritis, Knee Type of study: Etiology_studies Limits: Aged / Aged80 / Female / Humans / Male / Middle aged Language: En Journal: J Orthop Res Year: 2015 Type: Article Affiliation country: Japan

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stem Cells / Cartilage, Articular / Bone Malalignment / Chondrocytes / Osteoarthritis, Knee Type of study: Etiology_studies Limits: Aged / Aged80 / Female / Humans / Male / Middle aged Language: En Journal: J Orthop Res Year: 2015 Type: Article Affiliation country: Japan