Thermal aggregation behaviour of soy protein: characteristics of different polypeptides and sub-units.
J Sci Food Agric
; 96(4): 1121-31, 2016 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-25801436
ABSTRACT
BACKGROUND:
Due to the differences in structure and composition of glycinin and ß-conglycinin, they exhibit different characteristics during heat treatment. In present study, the thermal aggregation behaviour of glycinin, ß-conglycinin and their isolated sub-units was investigated at pH 7.0.RESULTS:
Acidic polypeptides, basic polypeptides, αα' and ß sub-units of soy protein were denatured during the isolation process. The degree of aggregation of protein fractions after heat treatment was in the order denatured basic polypeptides > native glycinin > denatured ß sub-unit > native ß-conglycinin > denatured acidic polypeptides > denatured αα' sub-units. Glycinin, ß-conglycinin, acidic polypeptides and αα'/ß sub-units exhibited different changing trends of surface hydrophobicity with increasing temperature. The αα' sub-units showed higher ability to suppress thermal aggregation of basic polypeptides than ß sub-units during heat treatment. The ß sub-units were shown to form soluble aggregates with glycinin after heating.CONCLUSION:
The interaction mechanism of αα' and ß sub-units heated with basic polypeptides was proposed. For the ß sub-units-basic polypeptides mixed system, more hydrophobic chains were binding together and buried inside during heat treatment, which resulted in lower surface hydrophobicity. The αα' sub-units-basic polypeptides mixed system was considered to be a stable system with higher surface hydrophobicity after being heated.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Soybean Proteins
Limits:
Humans
Language:
En
Journal:
J Sci Food Agric
Year:
2016
Type:
Article