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Kidney injury molecule-1 expression in human kidney transplants with interstitial fibrosis and tubular atrophy.
Nogare, Aline Lima; Veronese, Francisco Veríssimo; Carpio, Virna Nowotny; Montenegro, Rosangela Munhoz; Pedroso, José Alberto; Pegas, Karla Laís; Gonçalves, Luiz Felipe; Manfro, Roberto Ceratti.
Affiliation
  • Nogare AL; Post-Graduate Medical Sciences Program, School of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. alnogare@gmail.com.
  • Veronese FV; Post-Graduate Medical Sciences Program, School of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. fjvveronese@gmail.com.
  • Carpio VN; Renal Transplant Unit, Division of Nephrology, Hospital de Clínicas de Porto Alegre, Post-Graduate Medical Sciences Program, School of Medicine, Federal University of Rio Grande do Sul 2350, Ramiro Barcelos St. Room 2030, Zip Code: 90035-903, Porto Alegre, RS, Brazil. fjvveronese@gmail.com.
  • Montenegro RM; Post-Graduate Medical Sciences Program, School of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. virna.carpio@gmail.com.
  • Pedroso JA; Renal Transplant Unit, Division of Nephrology, Hospital de Clínicas de Porto Alegre, Post-Graduate Medical Sciences Program, School of Medicine, Federal University of Rio Grande do Sul 2350, Ramiro Barcelos St. Room 2030, Zip Code: 90035-903, Porto Alegre, RS, Brazil. rosangelamontenegro@yahoo.com.b
  • Pegas KL; Renal Transplant Unit, Division of Nephrology, Hospital de Clínicas de Porto Alegre, Post-Graduate Medical Sciences Program, School of Medicine, Federal University of Rio Grande do Sul 2350, Ramiro Barcelos St. Room 2030, Zip Code: 90035-903, Porto Alegre, RS, Brazil. josealbertopedroso@gmail.com.
  • Gonçalves LF; Division of Pathology, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil. lfp.voy@terra.com.br.
  • Manfro RC; Post-Graduate Medical Sciences Program, School of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. luisfelipe@maededeus.com.br.
BMC Nephrol ; 16: 19, 2015 Feb 13.
Article in En | MEDLINE | ID: mdl-25884518
BACKGROUND: Kidney injury molecule-1 (KIM-1) is expressed in tubular epithelial cells after injury and may have a role in the development of renal graft fibrosis. In this study we evaluated the molecular and protein expressions of KIM-1 in dysfunctional allografts and also mRNA KIM-1 expression in urine as potential biomarkers of graft fibrosis. METHODS: Protein and mRNA levels in renal tissue and urinary sediment cells of 69 kidney transplant recipients that undertook for-cause graft biopsies were evaluated by immunohistochemistry and real-time polymerase chain reaction. The histopathology was classified according to the 2007 Banff schema. RESULTS: KIM-1 protein expression was increased in biopsies with interstitial fibrosis and tubular atrophy (IF/TA) compared with biopsies showing acute calcineurin inhibitor nephrotoxicity (CIN) (P <0.05). Kidney tissue KIM-1 mRNA signaling (in) was increased in biopsies with IF/TA compared with all other groups (P <0.05). In the urine cells KIM-1 mRNA was also increased in patients with IF/TA compared with patients with acute CIN (P <0.05). Significant correlations were found between KIM-1 protein and mRNA levels in tissue, between mRNA expressions in tissue and urine and between protein tissue expression and gene expression in the urine. CONCLUSIONS: KIM-1 seems to be a marker of kidney graft fibrosis. Urinary KIM-1 mRNA may become a useful non-invasive biomarker of the injuries that can trigger intra-graft fibrotic processes, such as interstitial fibrosis and tubular atrophy.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Receptors, Virus / RNA, Messenger / Membrane Glycoproteins / Gene Expression Regulation / Kidney Transplantation / Graft Rejection / Kidney Tubules Type of study: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: BMC Nephrol Journal subject: NEFROLOGIA Year: 2015 Type: Article Affiliation country: Brazil

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Receptors, Virus / RNA, Messenger / Membrane Glycoproteins / Gene Expression Regulation / Kidney Transplantation / Graft Rejection / Kidney Tubules Type of study: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: BMC Nephrol Journal subject: NEFROLOGIA Year: 2015 Type: Article Affiliation country: Brazil