Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice.
Biomed Environ Sci
; 28(4): 272-80, 2015 Apr.
Article
in En
| MEDLINE
| ID: mdl-25966753
ABSTRACT
OBJECTIVE:
To determine the ability of grape seed proanthocyanidin extract (GSPE) in alleviating arsenic-induced reproductive toxicity.METHODS:
Sixty male Kunming mice received the following treatments by gavage normal saline solution (control); arsenic trioxide (ATO; 4 mg/kg); GSPE (400 mg/kg); ATO+GSPE (100 mg/kg); ATO+GSPE (200 mg/kg) and ATO+GSPE (400 mg/kg). Thereafter, the mice were sacrificed and weighed, and the testis was examined for pathological changes. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), heme oxygenase 1 (HO1), glutathione S-transferase (GST), NAD(P)H dehydrogenase, and quinone 1 (NQO1) expression in the testis was detected by real-time PCR. Superoxide dismutase (SOD), glutathione (GSH), total antioxidative capability (T-AOC), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), and reproductive indexes were analyzed.RESULTS:
ATO-treated mice showed a significantly decreased sperm count and testis somatic index and activity levels of SOD, GSH, and T-AOC than control group. Compared to the ATO-treated group, ATO +GSPE group showed recovery of the measured parameters. Mice treated with ATO+high-dose GSPE showed the highest level of mRNA expression of Nrf2, HO, NQO1, and GST.CONCLUSION:
GSPE alleviates oxidative stress damage in mouse testis by activating Nrf2 signaling, thus counteracting arsenic-induced reproductive toxicity.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arsenic
/
Testis
/
Signal Transduction
/
Proanthocyanidins
/
NF-E2-Related Factor 2
/
Grape Seed Extract
Limits:
Animals
Language:
En
Journal:
Biomed Environ Sci
Journal subject:
SAUDE AMBIENTAL
Year:
2015
Type:
Article
Affiliation country:
China