Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition.
RNA Biol
; 12(7): 690-700, 2015.
Article
in En
| MEDLINE
| ID: mdl-25970317
ABSTRACT
Treatment of leukemia cells with 1,25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22-3p and let-7e-5p.
Key words
AGO2, argonaute 2; AML; AML, acute myeloid leukemia; ECL methods, enhanced chemiluminescence methods; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GFP, green fluorescent protein; HPCs, haematopoietic progenitor cells; KPNA2, karyopherin α, 2; NBT assay, nitroblue tetrazolium assay; PLK1; PLK1, polo-like kinase 1; PMSF, phenylmethylsulfonyl fluoride; RAB10, member RAS oncogene family 10; RAB5C, member RAS oncogene family 5C; RT-qPCR, quantitative reverse transcription polymerase chain reaction; SF2A1, splicing factor 2A1; TFs, transcription factors; VitD3, 1,25-dihydroxyvitamin D3; miRNAs, microRNAs; microRNAs; myeloid differentiation; ribosomal/polysomal fractions
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Messenger
/
Monocytes
/
Cell Differentiation
/
RNA Processing, Post-Transcriptional
/
Granulocyte Precursor Cells
/
Gene Regulatory Networks
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
RNA Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2015
Type:
Article
Affiliation country:
Italy