Validation of the 6 Hz refractory seizure mouse model for intracerebroventricularly administered compounds.
Epilepsy Res
; 115: 67-72, 2015 Sep.
Article
in En
| MEDLINE
| ID: mdl-26220379
ABSTRACT
The six hertz (6 Hz) refractory seizure model is considered an indispensable chain of the Anticonvulsant Screening Project. We here describe an adapted protocol using the intracerebroventricular (i.c.v.) delivery route, which will allow researchers to perform targetvalidation or proof-of-principle studies using promising compounds with unknown or limited blood-brain barrier permeability (e.g. neuropeptides and peptidomimetics) in this model. Seizures were induced by single application of a current intensity of 49 mA to i.c.v.-implanted NMRI mice using an ECT Unit 57800 Ugo Basile stimulator. By applying these key parameters, c-Fos immunohistochemistry revealed the recruitment of the dentate gyrus, ratifying this model as a valuable tool for testing i.c.v. administered compounds against therapy-resistant seizures. This finding was further strengthened, since i.c.v. administration of levetiracetam suppressed 6 Hz-evoked seizure severity but sodium phenytoin did not. We also propose to use "seizure duration" as an alternative, accurate parameter to express the results within this model.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Seizures
/
Blood-Brain Barrier
/
Disease Models, Animal
/
Electric Stimulation
/
Anticonvulsants
Type of study:
Guideline
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Epilepsy Res
Journal subject:
CEREBRO
/
NEUROLOGIA
Year:
2015
Type:
Article