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Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations.
Sato, Masahiro; Koriyama, Miyu; Watanabe, Satoshi; Ohtsuka, Masato; Sakurai, Takayuki; Inada, Emi; Saitoh, Issei; Nakamura, Shingo; Miyoshi, Kazuchika.
Affiliation
  • Sato M; Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890-8544, Japan. masasato@ms.kagoshima-u.ac.jp.
  • Koriyama M; Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan. k3326891@kadai.jp.
  • Watanabe S; Animal Genome Research Unit, Division of Animal Science, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan. kettle@affrc.go.jp.
  • Ohtsuka M; Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa 259-1193, Japan. masato@is.icc.u-tokai.ac.jp.
  • Sakurai T; Department of Cardiovascular Research, Graduate school of Medicine, Shinshu University, Nagano 390-8621, Japan. tsakurai@shinshu-u.ac.jp.
  • Inada E; Department of Pediatric Dentistry, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan. inada@dent.kagoshima-u.ac.jp.
  • Saitoh I; Division of Pediatric Dentistry, Department of Oral Health Sciences, Course for Oral Life Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan. isaito@dent.niigata-u.ac.jp.
  • Nakamura S; Division of Biomedical Engineering, National Defense Medical College Research Institute, Saitama 359-8513, Japan. snaka@ndmc.ac.jp.
  • Miyoshi K; Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan. kmiyoshi@agri.kagoshima-u.ac.jp.
Int J Mol Sci ; 16(8): 17838-56, 2015 Aug 03.
Article in En | MEDLINE | ID: mdl-26247938
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oocytes / RNA, Messenger / Gene Silencing / INDEL Mutation / Galactosyltransferases / Mosaicism Type of study: Etiology_studies Limits: Animals / Female / Humans Language: En Journal: Int J Mol Sci Year: 2015 Type: Article Affiliation country: Japan
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oocytes / RNA, Messenger / Gene Silencing / INDEL Mutation / Galactosyltransferases / Mosaicism Type of study: Etiology_studies Limits: Animals / Female / Humans Language: En Journal: Int J Mol Sci Year: 2015 Type: Article Affiliation country: Japan