Comparative Proteomic Analysis of Wild-Type and SAP Domain Mutant Foot-and-Mouth Disease Virus-Infected Porcine Cells Identifies the Ubiquitin-Activating Enzyme UBE1 Required for Virus Replication.

Zhu, Zixiang; Yang, Fan; Zhang, Keshan; Cao, Weijun; Jin, Ye; Wang, Guoqing; Mao, Ruoqing; Li, Dan; Guo, Jianhong; Liu, Xiangtao; Zheng, Haixue

Zhu, Zixiang; Yang, Fan; Zhang, Keshan; Cao, Weijun; Jin, Ye; Wang, Guoqing; Mao, Ruoqing; Li, Dan; Guo, Jianhong; Liu, Xiangtao; Zheng, Haixue.

Affiliation

Zhu Z; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Yang F; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Zhang K; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Cao W; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Jin Y; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Wang G; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Mao R; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Li D; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Guo J; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Liu X; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.
Zheng H; State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, China.

J Proteome Res ; 14(10): 4194-206, 2015 Oct 02.

Article in En | MEDLINE | ID: mdl-26354183

Subject(s)

Protein Sorting Signals; Proteome/isolation & purification; Proteomics/methods; Ubiquitin-Activating Enzymes/chemistry; Viral Proteins/chemistry; Animals; Cell Line; Chromatography, Liquid; Epithelial Cells/metabolism; Epithelial Cells/pathology; Epithelial Cells/virology; Foot-and-Mouth Disease Virus/genetics; Foot-and-Mouth Disease Virus/metabolism; Host-Pathogen Interactions; Mutation; Peptides/analysis; Protein Structure, Tertiary; Proteolysis; Proteomics/instrumentation; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining and Labeling/methods; Swine; Trypsin/chemistry; Ubiquitin-Activating Enzymes/antagonists & inhibitors; Ubiquitin-Activating Enzymes/genetics; Ubiquitin-Activating Enzymes/metabolism; Viral Proteins/genetics; Viral Proteins/metabolism; Virus Replication/genetics

Key words

SAP domain; foot-and-mouth disease virus; iTRAQ; pathway analysis; quantitative proteomics

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Proteins / Protein Sorting Signals / Proteome / Proteomics / Ubiquitin-Activating Enzymes Limits: Animals Language: En Journal: J Proteome Res Journal subject: BIOQUIMICA Year: 2015 Type: Article Affiliation country: China

Fulltext

XML

PubMed Links

Search on Google