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Intestinal stem cell growth and differentiation on a tubular scaffold with evaluation in small and large animals.
Shaffiey, Shahab A; Jia, Hongpeng; Keane, Timothy; Costello, Cait; Wasserman, Deena; Quidgley, Maria; Dziki, Jenna; Badylak, Stephen; Sodhi, Chhinder P; March, John C; Hackam, David J.
Affiliation
  • Shaffiey SA; Division of Pediatric Surgery, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15217, USA.
  • Jia H; Division of Pediatric Surgery, Department of Surgery, Johns Hopkins University, 600 N Wolfe Street, Baltimore, MD 21287, USA.
  • Keane T; McGowan Institute for Regenerative Medicine, 450 Technology Drive Suite 300, Pittsburgh, PA 15219, USA.
  • Costello C; Department of Biological & Environmental Engineering, Cornell University, Ithaca, NY 14850, USA.
  • Wasserman D; Division of Pediatric Surgery, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15217, USA.
  • Quidgley M; Division of Pediatric Surgery, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15217, USA.
  • Dziki J; McGowan Institute for Regenerative Medicine, 450 Technology Drive Suite 300, Pittsburgh, PA 15219, USA.
  • Badylak S; McGowan Institute for Regenerative Medicine, 450 Technology Drive Suite 300, Pittsburgh, PA 15219, USA.
  • Sodhi CP; Division of Pediatric Surgery, Department of Surgery, Johns Hopkins University, 600 N Wolfe Street, Baltimore, MD 21287, USA.
  • March JC; Department of Biological & Environmental Engineering, Cornell University, Ithaca, NY 14850, USA.
  • Hackam DJ; Division of Pediatric Surgery, Department of Surgery, Johns Hopkins University, 600 N Wolfe Street, Baltimore, MD 21287, USA.
Regen Med ; 11(1): 45-61, 2016 Jan.
Article in En | MEDLINE | ID: mdl-26395928
ABSTRACT

AIMS:

To investigate the growth and differentiation of intestinal stem cells on a novel tubular scaffold in vitro and in vivo. MATERIALS &

METHODS:

Intestinal progenitor cells from mice or humans were cultured with myofibroblasts, macrophages and/or bacteria, and evaluated in mice via omental implantation. Mucosal regeneration was evaluated in dogs after rectal mucosectomy followed by scaffold implantation.

RESULTS:

Intestinal progenitor cells differentiated into crypt-villi structures on the scaffold. Differentiation and scaffold coverage was enhanced by coculture with myofibroblasts, macrophages and probiotic bacteria, while the implanted scaffolds enhanced mucosal regeneration in the dog rectum.

CONCLUSION:

Intestinal stem cell growth and differentiation on a novel tubular scaffold is enhanced through addition of cellular and microbial components, as validated in mice and dogs.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stem Cells / Cell Differentiation / Tissue Scaffolds / Intestine, Small Limits: Animals Language: En Journal: Regen Med Year: 2016 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stem Cells / Cell Differentiation / Tissue Scaffolds / Intestine, Small Limits: Animals Language: En Journal: Regen Med Year: 2016 Type: Article Affiliation country: United States