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A strategy for dissecting the architectures of native macromolecular assemblies.
Shi, Yi; Pellarin, Riccardo; Fridy, Peter C; Fernandez-Martinez, Javier; Thompson, Mary K; Li, Yinyin; Wang, Qing Jun; Sali, Andrej; Rout, Michael P; Chait, Brian T.
Affiliation
  • Shi Y; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, USA.
  • Pellarin R; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
  • Fridy PC; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA.
  • Fernandez-Martinez J; California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, California, USA.
  • Thompson MK; Institut Pasteur, Paris, France.
  • Li Y; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.
  • Wang QJ; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.
  • Sali A; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.
  • Rout MP; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York, USA.
Nat Methods ; 12(12): 1135-8, 2015 Dec.
Article in En | MEDLINE | ID: mdl-26436480
ABSTRACT
It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Conformation / Mass Spectrometry / Models, Molecular / Multiprotein Complexes Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2015 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Conformation / Mass Spectrometry / Models, Molecular / Multiprotein Complexes Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2015 Type: Article Affiliation country: United States