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A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis.
Zhai, Jixian; Bischof, Sylvain; Wang, Haifeng; Feng, Suhua; Lee, Tzuu-Fen; Teng, Chong; Chen, Xinyuan; Park, Soo Young; Liu, Linshan; Gallego-Bartolome, Javier; Liu, Wanlu; Henderson, Ian R; Meyers, Blake C; Ausin, Israel; Jacobsen, Steven E.
Affiliation
  • Zhai J; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
  • Bischof S; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
  • Wang H; Haixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry University, Fuzhou 350002, China; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
  • Feng S; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
  • Lee TF; Department of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19716, USA.
  • Teng C; Department of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19716, USA.
  • Chen X; Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095, USA.
  • Park SY; Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA.
  • Liu L; Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA.
  • Gallego-Bartolome J; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA; Plant Biology Laboratory and Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
  • Liu W; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
  • Henderson IR; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA.
  • Meyers BC; Department of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19716, USA.
  • Ausin I; Haixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: israelausin@fafu.edu.cn.
  • Jacobsen SE; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, USA; Howard Hughes Medical Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA. Electronic address: jacobsen@ucla.edu.
Cell ; 163(2): 445-55, 2015 Oct 08.
Article in En | MEDLINE | ID: mdl-26451488
ABSTRACT
RNA-directed DNA methylation in Arabidopsis thaliana is driven by the plant-specific RNA Polymerase IV (Pol IV). It has been assumed that a Pol IV transcript can give rise to multiple 24-nt small interfering RNAs (siRNAs) that target DNA methylation. Here, we demonstrate that Pol IV-dependent RNAs (P4RNAs) from wild-type Arabidopsis are surprisingly short in length (30 to 40 nt) and mirror 24-nt siRNAs in distribution, abundance, strand bias, and 5'-adenine preference. P4RNAs exhibit transcription start sites similar to Pol II products and are featured with 5'-monophosphates and 3'-misincorporated nucleotides. The 3'-misincorporation preferentially occurs at methylated cytosines on the template DNA strand, suggesting a co-transcriptional feedback to siRNA biogenesis by DNA methylation to reinforce silencing locally. These results highlight an unusual mechanism of Pol IV transcription and suggest a "one precursor, one siRNA" model for the biogenesis of 24-nt siRNAs in Arabidopsis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / RNA, Plant / RNA, Small Interfering Language: En Journal: Cell Year: 2015 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / RNA, Plant / RNA, Small Interfering Language: En Journal: Cell Year: 2015 Type: Article Affiliation country: United States