Proteomics in the genome engineering era.
Proteomics
; 16(2): 177-87, 2016 Jan.
Article
in En
| MEDLINE
| ID: mdl-26510734
ABSTRACT
Genome engineering experiments used to be lengthy, inefficient, and often expensive, preventing a widespread adoption of such experiments for the full assessment of endogenous protein functions. With the revolutionary clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 technology, genome engineering became accessible to the broad life sciences community and is now implemented in several research areas. One particular field that can benefit significantly from this evolution is proteomics where a substantial impact on experimental design and general proteome biology can be expected. In this review, we describe the main applications of genome engineering in proteomics, including the use of engineered disease models and endogenous epitope tagging. In addition, we provide an overview on current literature and highlight important considerations when launching genome engineering technologies in proteomics workflows.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Engineering
/
Proteomics
Limits:
Animals
/
Humans
Language:
En
Journal:
Proteomics
Journal subject:
BIOQUIMICA
Year:
2016
Type:
Article
Affiliation country:
Belgium