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Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems.
Shmakov, Sergey; Abudayyeh, Omar O; Makarova, Kira S; Wolf, Yuri I; Gootenberg, Jonathan S; Semenova, Ekaterina; Minakhin, Leonid; Joung, Julia; Konermann, Silvana; Severinov, Konstantin; Zhang, Feng; Koonin, Eugene V.
Affiliation
  • Shmakov S; Skolkovo Institute of Science and Technology, Skolkovo, 143025, Russia; NCBI, NLM, NIH, Bethesda, MD 20894, USA.
  • Abudayyeh OO; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological E
  • Makarova KS; NCBI, NLM, NIH, Bethesda, MD 20894, USA.
  • Wolf YI; NCBI, NLM, NIH, Bethesda, MD 20894, USA.
  • Gootenberg JS; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological E
  • Semenova E; Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Minakhin L; Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Joung J; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological E
  • Konermann S; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological E
  • Severinov K; Skolkovo Institute of Science and Technology, Skolkovo, 143025, Russia; Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA; Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia.
  • Zhang F; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological E
  • Koonin EV; NCBI, NLM, NIH, Bethesda, MD 20894, USA. Electronic address: koonin@ncbi.nlm.nih.gov.
Mol Cell ; 60(3): 385-97, 2015 Nov 05.
Article in En | MEDLINE | ID: mdl-26593719
Microbial CRISPR-Cas systems are divided into Class 1, with multisubunit effector complexes, and Class 2, with single protein effectors. Currently, only two Class 2 effectors, Cas9 and Cpf1, are known. We describe here three distinct Class 2 CRISPR-Cas systems. The effectors of two of the identified systems, C2c1 and C2c3, contain RuvC-like endonuclease domains distantly related to Cpf1. The third system, C2c2, contains an effector with two predicted HEPN RNase domains. Whereas production of mature CRISPR RNA (crRNA) by C2c1 depends on tracrRNA, C2c2 crRNA maturation is tracrRNA independent. We found that C2c1 systems can mediate DNA interference in a 5'-PAM-dependent fashion analogous to Cpf1. However, unlike Cpf1, which is a single-RNA-guided nuclease, C2c1 depends on both crRNA and tracrRNA for DNA cleavage. Finally, comparative analysis indicates that Class 2 CRISPR-Cas systems evolved on multiple occasions through recombination of Class 1 adaptation modules with effector proteins acquired from distinct mobile elements.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ribonucleases / Bacteria / Bacterial Proteins / RNA, Bacterial / Evolution, Molecular / CRISPR-Cas Systems Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2015 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ribonucleases / Bacteria / Bacterial Proteins / RNA, Bacterial / Evolution, Molecular / CRISPR-Cas Systems Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2015 Type: Article Affiliation country: United States