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Spatiotemporal Analysis of the Genetic Diversity of Seal Influenza A(H10N7) Virus, Northwestern Europe.
Bodewes, Rogier; Zohari, Siamak; Krog, Jesper S; Hall, Matthew D; Harder, Timm C; Bestebroer, Theo M; van de Bildt, Marco W G; Spronken, Monique I; Larsen, Lars E; Siebert, Ursula; Wohlsein, Peter; Puff, Christina; Seehusen, Frauke; Baumgärtner, Wolfgang; Härkönen, Tero; Smits, Saskia L; Herfst, Sander; Osterhaus, Albert D M E; Fouchier, Ron A M; Koopmans, Marion P; Kuiken, Thijs.
Affiliation
  • Bodewes R; Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands rogier.bodewes@gmail.com.
  • Zohari S; Department of Virology, Immunobiology and Parasitology, National Veterinary Institute, Uppsala, Sweden.
  • Krog JS; National Veterinary Institute, Technical University of Denmark, Frederiksberg, Denmark.
  • Hall MD; Centre for Immunity, Infection and Evolution, University of Edinburgh, Edinburgh, United Kingdom.
  • Harder TC; Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Greifswald Insel-Riems, Germany.
  • Bestebroer TM; Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.
  • van de Bildt MWG; Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.
  • Spronken MI; Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.
  • Larsen LE; National Veterinary Institute, Technical University of Denmark, Frederiksberg, Denmark.
  • Siebert U; Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, Büsum, Germany.
  • Wohlsein P; Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
  • Puff C; Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
  • Seehusen F; Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
  • Baumgärtner W; Department of Pathology, University of Veterinary Medicine Hannover, Hannover, Germany.
  • Härkönen T; Department of Environmental Research and Monitoring, Swedish Museum of Natural History, Stockholm, Sweden.
  • Smits SL; Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.
  • Herfst S; Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.
  • Osterhaus ADME; Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.
  • Fouchier RAM; Artemis One Health, Utrecht, the Netherlands.
  • Koopmans MP; Research Centre for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Hannover, Germany.
  • Kuiken T; Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.
J Virol ; 90(9): 4269-4277, 2016 May.
Article in En | MEDLINE | ID: mdl-26819311
UNLABELLED: Influenza A viruses are major pathogens for humans, domestic animals, and wildlife, and these viruses occasionally cross the species barrier. In spring 2014, increased mortality of harbor seals (Phoca vitulina), associated with infection with an influenza A(H10N7) virus, was reported in Sweden and Denmark. Within a few months, this virus spread to seals of the coastal waters of Germany and the Netherlands, causing the death of thousands of animals. Genetic analysis of the hemagglutinin (HA) and neuraminidase (NA) genes of this seal influenza A(H10N7) virus revealed that it was most closely related to various avian influenza A(H10N7) viruses. The collection of samples from infected seals during the course of the outbreak provided a unique opportunity to follow the adaptation of the avian virus to its new seal host. Sequence data for samples collected from 41 different seals from four different countries between April 2014 and January 2015 were obtained by Sanger sequencing and next-generation sequencing to describe the molecular epidemiology of the seal influenza A(H10N7) virus. The majority of sequence variation occurred in the HA gene, and some mutations corresponded to amino acid changes not found in H10 viruses isolated from Eurasian birds. Also, sequence variation in the HA gene was greater at the beginning than at the end of the epidemic, when a number of the mutations observed earlier had been fixed. These results imply that when an avian influenza virus jumps the species barrier from birds to seals, amino acid changes in HA may occur rapidly and are important for virus adaptation to its new mammalian host. IMPORTANCE: Influenza A viruses are major pathogens for humans, domestic animals, and wildlife. In addition to the continuous circulation of influenza A viruses among various host species, cross-species transmission of influenza A viruses occurs occasionally. Wild waterfowl and shorebirds are the main reservoir for most influenza A virus subtypes, and spillover of influenza A viruses from birds to humans or other mammalian species may result in major outbreaks. In the present study, various sequencing methods were used to elucidate the genetic changes that occurred after the introduction and subsequent spread of an avian influenza A(H10N7) virus among harbor seals of northwestern Europe by use of various samples collected during the outbreak. Such detailed knowledge of genetic changes necessary for introduction and adaptation of avian influenza A viruses to mammalian hosts is important for a rapid risk assessment of such viruses soon after they cross the species barrier.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genetic Variation / Orthomyxoviridae Infections / Phoca / Influenza A Virus, H10N7 Subtype / Spatio-Temporal Analysis Type of study: Risk_factors_studies Limits: Animals Country/Region as subject: Europa Language: En Journal: J Virol Year: 2016 Type: Article Affiliation country: Netherlands

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genetic Variation / Orthomyxoviridae Infections / Phoca / Influenza A Virus, H10N7 Subtype / Spatio-Temporal Analysis Type of study: Risk_factors_studies Limits: Animals Country/Region as subject: Europa Language: En Journal: J Virol Year: 2016 Type: Article Affiliation country: Netherlands